Journal: Genetics

Article Title: Molecular Characterization of Vegetative Incompatibility Genes That Restrict Hypovirus Transmission in the Chestnut Blight Fungus Cryphonectria parasitica

doi: 10.1534/genetics.111.133983

Figure Lengend Snippet: Cryphonectria parasitica strains used in this study

Article Snippet: The plates were incubated at 30° for 24 hr or until the paired colony margins overlapped by ∼1 mm at which time the membrane with the paired colonies was transferred to PDA+hyg+G418 [PDA, 30 μg/ml hygromycin B (Roche, Laval, QC, Canada), and 20 μg/ml G418 (an analog of neomycin: BioShop Canada, Burlington, ON, Canada)].

Techniques:

Journal: Virology

Article Title: Retroviral recombination is nonrandom and sequence dependent.

doi: 10.1006/viro.1998.9052

Figure Lengend Snippet: FIG. 2. Establishment of doubly infected cell clones and protocol for one cycle of replication. In step 1, each vector DNA was transfected separately into DSDh helper cells. Selection with the appropriate drug was performed, and resistant cells were pooled separately. Virus was harvested from hygromycin resistant (Hygr) step 1 cells and was used to infect fresh DSDh helper cells (step 2a). Hygr cells were cloned and were then infected with virus harvested from G418-resistant (G418r) step 1 cells (step 2b). Finally, selection with hygromycin and G418 was performed, and doubly resistant cell clones were isolated. Virus was isolated from doubly infected helper cell clones and was used to infect D-17 target cells in order to achieve one cycle of replication (step 3).

Article Snippet: Selection with G418 sulfate (a neomycin analog; Gibco BRL, Grand Island, NY) was performed at 400 mg/ml.

Techniques: Infection, Clone Assay, Plasmid Preparation, Transfection, Selection, Virus, Isolation